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目的:建立测定复方异丙嗪甘草口服溶液中盐酸异丙嗪和甘草酸含量的高效液相色谱法。方法:采用Thermo BDS C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇-冰醋酸-0.2 mol·L-1醋酸铵溶液(58∶1∶41)为流动相,流速为1.0 ml·min-1,检测波长为250 nm,柱温为室温,进样量为10μl。结果:盐酸异丙嗪、甘草酸分别在0.079 4~0.317 6 mg·ml-1(r=0.999 9)、0.060 3~0.241 1 mg·ml-1(r=0.999 9)范围内线性关系良好,平均回收率分别为99.61%(RSD=0.32%,n=9)、99.30%(RSD=0.59%,n=9)。结论:该方法简便准确、重复性好,可用于控制该制剂质量。
Objective: To establish a HPLC method for the determination of promethazine and glycyrrhizic acid in the oral solution of compound promethazine and licorice. Methods: The mobile phase was Thermo BDS C18 (250 mm × 4.6 mm, 5 μm) with methanol-glacial acetic acid-0.2 mol·L-1 ammonium acetate solution (58:1:41) at a flow rate of 1.0 ml · min -1, the detection wavelength was 250 nm, the column temperature was room temperature, the injection volume was 10μl. Results: Promethazine hydrochloride and glycyrrhizic acid showed a good linear relationship in the range of 0.079 4-0.317 6 mg · ml -1 (r = 0.999 9) and 0.060 3-0.241 mg · ml-1 (r = 0.999 9) The average recoveries were 99.61% (RSD = 0.32%, n = 9) and 99.30% (RSD = 0.59%, n = 9), respectively. Conclusion: The method is simple and accurate, reproducible, and can be used to control the quality of the preparation.