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目的:观察高压氧治疗新生大鼠缺氧缺血性脑损伤(HIBI)后脑内强啡肽A1-13水平的动态变化规律。方法:7d龄SD大鼠左侧颈总动脉结扎联合低氧吸收形成HIBI,在HIBI后施以高压氧治疗(A),并设HIBI+常氧高压氮组(B)、单纯HIBI组(C)、假手术组(D)和正常组(E)。观察各组处理后不同时间左侧皮层、海马、纹状体和血浆强啡肽A1-13免疫活性物质(ir-DynA1-13)含量的变化。结果:ir-DyA1-13含量,1次处理后与B组比较,A组皮层、海马含量均显著降低(P<0.01),但皮层与D组比较仍显著高(P<0.05);7次处理后与B组比较,A组皮层、海马含量仍显著降低(P<0.05)。1次处理后与B或C组比较,A组血浆ir-DynA1-13含量显著降低。结论:强啡肽A1-13参与新生大鼠HIBI的病理生理过程,通过逆转中枢强啡肽A1-13水平的异常改变是高压氧治疗新生大鼠HIBI的机制之一。
OBJECTIVE: To observe the dynamic changes of brain Dynorphin A1-13 level after hyperbaric oxygen treatment in neonatal rats with hypoxic-ischemic brain damage (HIBI). Methods: HIBI was induced by left common carotid artery ligation and hypoxia uptake in 7-day-old SD rats and hyperbaric oxygen therapy after HIBI (A). HIBI + normoxic nitrogen group (B), HIBI-only group (C) , Sham operation group (D) and normal group (E). The changes of the immunoreactive substance (ir-DynA1-13) of left ventricle, hippocampus, striatum and plasma dynorphin A1-13 were observed at different time points after treatment. Results: Compared with group B, the content of ir-DyA1-13 in group A was significantly lower than that in group B (P <0.01), but the level of ir-DyA1-13 in group C was still significantly higher than that in group B (P <0.05) Compared with group B, the content of cortex and hippocampus in group A still decreased significantly (P <0.05). Compared with group B or C after 1 treatment, the content of ir-DynA1-13 in group A was significantly decreased. CONCLUSION: The dynorphin A1-13 is involved in the pathophysiological process of HIBI in neonatal rats. One of the mechanisms of hyperbaric oxygen treatment of HIBI in neonatal rats by reversing the abnormal changes of the concentration of norepinephrine A1-13 was studied.