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近年来,国外大量采用体外细胞培养技术进行垂体细胞分泌功能的研究,使用限制的无血清培养液对垂体细胞进行原代培养,可消除有血清培养时测定工作的不便和误差.本实验采用多种酶综合消化,得到分散、形态完整的垂体前叶和后叶细胞,进行无血清原代培养,观察细胞形态,并测定其分泌功能. 实验选用健康成年雄性Sprague-Dawley大鼠,体重180-250g。无血清培养体系包括1:1混合的DMEM和Ham’s F-12的人工合成培养液,并添加胰岛素5μg/ml、人转铁蛋白10μg/ml、亚硒酸钠
In recent years, a large number of foreign countries in vitro cell culture technology for pituitary cell secretion research, the use of limited serum-free medium for primary culture of pituitary cells can eliminate the inconvenience and error when the determination of serum culture.This experiment used more The enzyme synthesis digestion, to get decentralized, morphologically intact anterior pituitary and posterior lobe cells, serum-free primary culture, observe the cell morphology, and determine its secretion function.Experiment Healthy healthy male Sprague-Dawley rats weighing 180- 250g. The serum-free culture system consisted of a 1: 1 synthetic DMEM and Ham’s F-12 artificial culture medium supplemented with insulin 5 μg / ml, human transferrin 10 μg / ml, sodium selenite