论文部分内容阅读
目的探讨钙黏蛋白E(E-cadherin)、桥粒芯糖蛋白(2DSG2)、磷酸化Akt(pAkt)和转录因子Snail在前列腺癌发病进程中的作用;阐明E-cadherin、DSG2、pAkt和Snail对前列腺癌发病进程的影响及在临床预后中的作用。方法组织芯片法检测E-cadherin、DSG2、pAkt和Snail在前列腺癌组织中的表达情况。结果前列腺癌组织中E-cadherin表达明显低于正常前列腺组织,细胞质和细胞核中均可观察到pAkt表达,且在肿瘤高分化区和低分化区内均有较高表达。E-cadherin和DSG2表达呈显著正相关。E-cadherin和DSG2表达下降提示前列腺癌患者血清前列腺特异性抗原(PSA)浓度含量增加,Gleason评分较高及病理分期更高。结论前列腺癌低分化细胞中钙黏蛋白表达降低,钙黏蛋白低表达提示肿瘤细胞侵袭性更强,转移发生率高。钙黏蛋白在前列腺癌发展中发挥关键作用,E-cadherin和DSG2有望成为侵袭性前列腺癌的预后因子。
Objective To investigate the role of E-cadherin, desmoglein (2DSG2), phosphorylated Akt (pAkt) and transcription factor Snail in the pathogenesis of prostate cancer. E-cadherin, DSG2, pAkt and Snail Effect on Prostate Cancer Pathogenesis and Its Clinical Prognosis. Methods Tissue microarray was used to detect the expression of E-cadherin, DSG2, pAkt and Snail in prostate cancer tissues. Results The expression of E-cadherin in prostate cancer tissue was significantly lower than that in normal prostate tissue. The expression of pAkt was observed in both cytoplasm and nucleus, and was highly expressed in well-differentiated and poorly differentiated tumors. There was a significant positive correlation between E-cadherin and DSG2 expression. Decreased expression of E-cadherin and DSG2 suggest that serum prostate-specific antigen (PSA) concentration is increased, Gleason score is higher and pathological stage is higher in patients with prostate cancer. Conclusions Decreased cadherin expression in poorly differentiated prostate cancer cells and low expression of cadherin suggest that tumor cells are more aggressive and have a higher incidence of metastasis. Cadherin plays a key role in the development of prostate cancer, and E-cadherin and DSG2 are expected to be prognostic factors for invasive prostate cancer.