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目的:通过大蒜素预处理,观察全脑缺血再灌注大鼠海马区ICAM-1的表达,从而探讨大蒜素的脑保护机制。方法:雄性Wistar大鼠30只,随机分为5组:假手术组、缺血再灌注组、缺血再灌注+大蒜素10、20、30 mg/kg组。采用四血管闭塞法制备大鼠全脑缺血再灌注模型,于再灌注24 h取出海马,硫堇染色观察海马组织的形态学改变,免疫组织化学染色测定海马CA1区ICAM-1免疫反应阳性细胞面积和积分光密度值。结果:通过给予大鼠全脑缺血8 min再灌注24 h处理,海马CA1区组织形态学改变显著,神经元密度明显降低;ICAM-1的表达显著增加。静脉给予大蒜素可使缺血再灌注海马组织形态学改变明显改善,存活神经元数目增加,ICAM-1表达显著较少。结论:大蒜素可以通过减少ICAM-1的表达抑制全脑缺血再灌注后的炎症损失从而发挥脑保护作用。
OBJECTIVE: To observe the ICAM-1 expression in hippocampus of rats with global cerebral ischemia / reperfusion by allicin pretreatment, and to explore the protective mechanism of allicin. Methods: Thirty male Wistar rats were randomly divided into 5 groups: sham-operation group, ischemia-reperfusion group, ischemia reperfusion + allicin 10, 20, 30 mg / kg group. The rat model of global cerebral ischemia-reperfusion was established by four-vessel occlusion. The hippocampus was removed 24 h after reperfusion and the morphological changes of hippocampus were observed by thionine staining. The expression of ICAM-1 immunoreactive cells in hippocampal CA1 area was detected by immunohistochemical staining Area and integral optical density values. Results: The histopathology of hippocampal CA1 region was significantly changed and the density of neurons was significantly decreased. The expression of ICAM-1 was significantly increased by giving the rats brain ischemia for 8 min and reperfusion for 24 h. Intraperitoneal administration of allicin can significantly improve the morphological changes of hippocampus after ischemia-reperfusion, and increase the number of surviving neurons, with significantly less ICAM-1 expression. CONCLUSION: Allicin can exert cerebral protective effect by decreasing the expression of ICAM-1 and inhibiting the loss of inflammation after global cerebral ischemia-reperfusion.