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目的 观察 IL - 2对 C6大鼠星形神经胶质瘤细胞增殖的影响 ,并从蛋白及 m RNA水平观察白细胞介素 - 2受体(IL - 2 R)α,β,γ 3条链在 C6神经胶质瘤细胞中的表达 .方法 应用细胞培养、MTT法、3H- Td R掺入法、免疫细胞化学方法和 RT- PCR技术 .结果 1IL - 2 (1× 10 4 ～ 1× 10 6 U·L- 1 )可显著促进 C6细胞的增殖 ;2 C6细胞中检测到 IL - 2 R的 β、γ2条链的免疫反应阳性物质 ;3IL- 2 Rβ,γ m RNA在C6细胞中均有表达 .结论 IL - 2可显著促进 C6神经胶质瘤细胞的增殖 ,C6细胞表达 IL- 2 Rβ、γ2条链的蛋白与 m RNA,可能介导 IL- 2增殖信号的传递 ,从而初步证实 IL- 2可直接作用于神经胶质细胞并进一步阐明了 IL- 2增殖信号的转导机制 ,为揭示细胞因子在神经系统中的普遍作用机制提供更多证据 .
Objective To observe the effect of IL - 2 on the proliferation of astrocytic glioma C6 glioma cells and to observe the expression of IL - 2R α, β, γ 3 chains in protein and m RNA level C6 glioma cells.Methods Cell culture, MTT assay, 3H-TdR incorporation, immunocytochemistry and RT-PCR were used to detect the expression of 1 IL-2 (1 × 10 4 ~ 1 × 10 6 U · L-1) could significantly promote the proliferation of C6 cells. The immunoreactive positive substances of β, γ2 chains of IL-2 R were detected in 2 C6 cells; 3IL-2 Rβ and γ m RNA were all found in C6 cells .Conclusion IL - 2 can significantly promote the proliferation of C6 glioma cells. C6 cells express the protein and m RNA of IL - 2 Rβ and γ2 chains, which may mediate the signal transduction of IL - 2 proliferation. - 2 can act directly on glial cells and further elucidate the transduction mechanism of IL - 2 proliferation signal, providing more evidence for revealing the general mechanism of action of cytokines in the nervous system.