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目的:建立同时测定不同产地葛根中3’-羟基葛根素、葛根素、3’-甲氧基葛根素、大豆苷和大豆苷元5个成分含量的UHPLC法。方法:采用Agela Venusil MP C18(100 mm×2.1 mm,3μm)色谱柱,以甲醇-0.1%甲酸梯度洗脱,检测波长250 nm。结果:3’-羟基葛根素、葛根素、3’-甲氧基葛根素、大豆苷和大豆苷元分别在12.41~248.24ng(r=0.9999)、58.82~1 176.47 ng(r=0.9997)、12.65~252.94 ng(r=0.9999)、12.14~242.82 ng(r=0.9998)、1.82~36.30 ng(r=0.9997)进样量范围内与峰面积呈良好的线性关系;平均加样回收率为99.03%~100.32%,RSD值为0.26%~1.37%。结论:本方法简便可靠、快速,可有效控制葛根的质量。
OBJECTIVE: To establish a UHPLC method for the simultaneous determination of 3-hydroxy puerarin, puerarin, 3’-methoxy puerarin, daidzin and daidzein in Pueraria lobata from different areas. Methods: A gradient of methanol-0.1% formic acid was used on a Agela Venusil MP C18 (100 mm × 2.1 mm, 3 μm) column with a detection wavelength of 250 nm. Results: The concentrations of 3’-hydroxy puerarin, puerarin, 3’-methoxy puerarin, daidzin and daidzein were in the range of 12.41 ~ 248.24ng (r = 0.9999), 58.82 ~ The average linear range was 12.65 ~ 252.94 ng (r = 0.9999), 12.14 ~ 242.82 ng (r = 0.9998) and 1.82 ~ 36.30 ng (r = 0.9997) % ~ 100.32%, RSD 0.26% ~ 1.37%. Conclusion: The method is simple, reliable and rapid, which can effectively control the quality of Pueraria.