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目的:研究Zn2+沉淀法纯化柿果多酚的最佳条件及其抑菌活性。方法:采用单因素及正交试验,得到最佳沉淀及转溶条件,并检测纯化后柿果多酚对8种常见致病菌的最小抑菌浓度。结果表明:柿果多酚沉淀最佳条件为m(柿果多酚)∶m(ZnCl2)=1∶5,pH6.8,时间25min,温度30℃;其转溶最佳条件为盐酸浓度2.5mol/L,料酸比1∶5g/mL,时间20min,温度30℃。在此条件下多酚得率12%,纯度92.6%。柿果多酚对大肠杆菌、金黄色葡萄球菌、鼠伤寒氏沙门氏菌、奇异变形杆菌、志贺氏痢疾杆菌、乙型溶血链球菌、肺炎链球菌及粪肠球菌最小抑菌质量浓度分别为0.1、0.05、0.1、0.2、0.8、1.6、1.6、0.05mg/mL。结论:Zn2+沉淀法可有效纯化柿果多酚,纯化后的柿果多酚有较强抑菌活性。
Objective: To study the optimum conditions and antibacterial activity of polyphenols purified by Zn2 + precipitation. Methods: Single factor and orthogonal experiments were used to obtain the optimum conditions for precipitation and solubilization. The minimum inhibitory concentration (MIC) of persimmon polyphenols on eight common pathogenic bacteria was tested. The results showed that the optimum conditions for persimmon polyphenols precipitation were m (persimmon polyphenols): m (ZnCl2) = 1: 5, pH6.8, time 25min, temperature 30 ℃; mol / L, acid ratio of 1: 5g / mL, time 20min, temperature 30 ℃. In this condition polyphenols yield 12%, purity 92.6%. The minimum inhibitory concentration of persimmon polyphenols on Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, Proteus mirabilis, Shigella dysenteriae, hemolytic streptococcus, Streptococcus pneumoniae and Enterococcus faecalis were 0.1, 0.05, 0.1, 0.2, 0.8, 1.6, 1.6, 0.05 mg / mL. Conclusion: Zn2 + precipitation method can effectively purify persimmon polyphenols, purified persimmon polyphenols have strong antibacterial activity.