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目的 观察牛初乳短链胰岛素样生长因子－１（ＢＣｔＩＧＦ－１）对糖尿病肾病的保护作用及探讨其可能的作用机制。方法采用生化法、放射免疫法、荧光分析法及逆转录．聚合酶链式反应（ＲＴ－ＰＣＲ）技术等进行研究。结果（１）糖尿病治疗组的空腹血糖、甘油三酯、果糖胺明显低于糖尿病对照组；（２）糖尿病治疗组２４ｈ尿白蛋白排泄率、２４ｈ尿量、２４ｈ内生肌酐清除率低于糖尿病对照组；（３）电镜观察大鼠肾脏病理切片发现ＢＣｔＩＧＦ－１对糖尿病大鼠肾脏损害有改善作用；（４）糖尿病对照组肾组织ＩＧＦ－１ｍＲＮＡ表达明显低于正常对照组，糖尿病对照组与糖尿病治疗组无明显异常；（５）糖尿病治疗组肾组织胶原含量及糖基化终末产物（ＡＧＥｓ）、尿血栓素Ｂ２／６－酮－前列环素１α（ＴＸＢ２／６－ｋｅｔｏ－ＰＧＦ１α）低于糖尿病对照组。结论（１）ＢＣｔＩＧＦ－１可以明显降低ＳＴＺ糖尿病大鼠的血糖、血脂；（２） ＢＣｔＩＧＦ－１不会引起肾皮质 ＩＧＦ－１ｍＲＮＡ表达的增加；（３） ＢＣｔＩＧＦ－１对 ＳＴＺ糖尿病大鼠肾脏有保护作用，其保护机制是由于ＢＣｔＩＧＦ－１对血糖、血脂的改善；此外，可能与其减少紧组织胶原及ＡＧＥｓ含量，改善前列腺素－血栓素合成的不平衡?
Objective To observe the protective effect of bovine colostrum short chain insulin-like growth factor-1 (BCtIGF-1) on diabetic nephropathy and to explore its possible mechanism. Methods Biochemical methods, radioimmunoassay, fluorescence analysis and reverse transcription. Polymerase chain reaction (RT-PCR) technology to study. Results (1) The fasting blood glucose, triglyceride and fructosamine in the diabetic group were significantly lower than those in the diabetic control group. (2) The urinary albumin excretion rate, 24-hour urine output and endogenous creatinine clearance rate in the diabetic group were lower than those in the diabetic group (4) The expression of IGF-1mRNA in kidney tissue of diabetic control group was significantly lower than that of normal control group, while the control group and (5) The content of collagen and the content of advanced glycosylation end products (AGEs), TXB2 / 6-keto-PGF1α in diabetes mellitus group were significantly higher than those in diabetic group Lower than the diabetic control group. (1) BCtIGF-1 can significantly reduce blood glucose and blood lipids in STZ diabetic rats; (2) BCtIGF-1 does not cause renal cortex IGF-1mRNA expression increased; (3) BCtIGF- Its protective mechanism is due to the improvement of blood glucose and blood lipid of BCtIGF-1. In addition, it may reduce the content of collagen and AGEs in tissue and improve the imbalance of prostaglandin-thromboxane synthesis.