最近几个实验室的研究人员报道:不用逆病毒,将基因直接注射入活体内,无需整合入宿主细胞基因组内也可在体内获得长期表达。与逆病毒枸建体相比,其优点为:①制备和实验具调控部件的质粒DNA构建体的技术更容易,且省时;②排除了逆病毒的致瘤性或重组内源性病毒感染的可能;③逆病毒介导的转化细胞稳定表达,但当希望停止其不良副作用时则不易。其方法主要有三种:①直接注射非感染性,非致癌性的质粒DNA,由脂质体、免疫脂质体和脂质体/红细胞膜杂交体包裹;②直接给新生大鼠膜腔内注射各种磷酸钙沉淀的基因序 Researchers from several laboratories recently reported that without retroviruses, direct gene injection into a living organism can achieve long-term in vivo expression without integration into the host cell genome. Compared with the retroviral construct, its advantages are: (1) The technique of preparing and assaying plasmid DNA constructs with regulated components is easier and time-saving; (2) exclusion of retroviral neoplastic or recombinant endogenous viral infection Of the possible; ③ retrovirus-mediated transformation of stable expression of cells, but when you want to stop the adverse side effects is not easy. There are three main ways: ① direct injection of non-infectious, non-carcinogenic plasmid DNA, liposomes, immunoliposomes and liposome / red cell membrane hybrid coating; ② directly to the newborn rat intracavity Various calcium phosphate precipitation gene order