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目的探讨基质金属蛋白酶14(MMP-14)在结直肠癌组织中的表达及临床意义。方法应用Western blot法检测8例新鲜结直肠癌和癌旁组织中MMP-14的表达;收集90例结直肠癌和30例癌旁组织石蜡包埋标本,应用免疫组织化学法检测MMP-14的表达。分析MMP-14在结直肠癌和癌旁组织中表达的差异及其与临床病理参数的关系。结果 MMP-14在结直肠癌组织中的表达高于癌旁正常组织(P<0.01);MMP-14蛋白的表达强度与结直肠癌的肿瘤浸润深度、淋巴转移和Dukes分期有显著相关性(P<0.05)。肿瘤是否伴有淋巴转移、浸润深度、Dukes分期及MMP-14表达水平与结直肠癌患者术后生存期长短密切相关(P<0.05);MMP-14表达可作为结直肠癌预后的独立指标(P<0.05)。结论 MMP-14在结直肠癌组织中的高表达可能与结直肠癌的发生、发展密切相关;MMP-14可作为结直肠癌患者独立预后指标。
Objective To investigate the expression and clinical significance of matrix metalloproteinase-14 (MMP-14) in colorectal cancer. Methods Western blot was used to detect the expression of MMP-14 in 8 cases of fresh colorectal cancer and paracancerous tissues; 90 cases of colorectal cancer and 30 cases of paracancerous tissues were collected and paraffin-embedded specimens were collected. Immunohistochemistry was used to detect the expression of MMP-14 expression. To analyze the difference of MMP-14 expression in colorectal cancer and paracancerous tissues and its relationship with clinicopathological parameters. Results The expression of MMP-14 in colorectal cancer tissue was higher than that in adjacent normal tissues (P <0.01). The expression of MMP-14 protein was significantly correlated with the depth of tumor invasion, lymphatic metastasis and Dukes stage in colorectal cancer P <0.05). The degree of invasion, depth of invasion, Dukes staging and MMP-14 expression in patients with colorectal cancer were significantly correlated with tumor survival (P <0.05). The expression of MMP-14 was an independent prognostic factor for colorectal cancer P <0.05). Conclusion The high expression of MMP-14 in colorectal cancer may be closely related to the occurrence and development of colorectal cancer. MMP-14 may be used as an independent prognostic factor in patients with colorectal cancer.