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目的:探讨阿糖胞苷联合冬凌草甲素诱导白血病细胞株U937凋亡的作用。方法:用阿糖胞苷和冬凌草甲素单药或两药联合处理U937细胞后,采用细胞计数检测细胞增殖,瑞氏染色观察细胞形态,流式细胞术分析细胞磷脂酰丝氨酸外翻情况和线粒体膜电位变化,蛋白印迹法检测凋亡相关分子表达情况。结果:阿糖胞苷和冬凌草甲素单药均能抑制U937细胞增殖,而两药联合抑制作用更加明显,两者能协同诱导U937细胞凋亡,并明显促进细胞磷脂酰丝氨酸外翻和线粒体跨膜电位下降;两药联合较单药组还能明显诱导Caspase-9、Caspase-3活化及PARP的剪切。结论:阿糖胞苷与冬凌草甲素能够协同抑制U937细胞增殖、诱导细胞凋亡,其机制可能是主要通过线粒体途径介导。
Objective: To investigate the effects of cytarabine combined with oridonin on apoptosis of leukemia cell line U937. Methods: U937 cells were treated with cytarabine and oridonin alone or in combination with two drugs. Cell proliferation was measured by cell counting. Wright’s stain was used to observe the cell morphology. Flow cytometry was used to analyze the cell phosphatidylserine valgus And mitochondrial membrane potential changes were detected by Western blotting apoptosis related molecules expression. Results: Both cytarabine and oridonin could inhibit the proliferation of U937 cells, but the combination of the two drugs was more obvious. Both of them could synergistically induce the apoptosis of U937 cells and significantly promote the cell phosphatidylserine valgus and Mitochondrial transmembrane potential decreased; two drugs combined with single drug group can significantly induce the activation of Caspase-9, Caspase-3 and PARP cleavage. CONCLUSION: Cytarabine and oridonin can synergistically inhibit the proliferation and induce apoptosis of U937 cells. The mechanism may be mainly mediated by mitochondria pathway.