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疣蝗分布广泛,是一种常见的害虫,主要危害农作物,但分子信息缺乏。我们采用高通量Illumina测序技术和从头组装的方法获得了疣蝗转录组unigenes,并鉴定了天然免疫相关信号通路基因。本研究共获得疣蝗转录组unigenes 86 756个,平均长度为670 bp,N50为1 033 bp。使用六大数据库Nr、Nt、Swiss-Prot、GO、COG和KEGG注释疣蝗转录组unigenes,分别对应有29 627,13 866,23 398,13 474,11 968和21 027个unigenes获得注释。本研究共鉴定319个天然免疫信号通路相关的unigenes,其中,参与Toll通路的unigenes 49个,参与免疫缺失(IMD)通路的unigenes 13个,参与JAK-STAT信号通路的unigenes 117个,参与MAPK-fly通路相关的unigenes 58个,参与黑化(Melanization)通路的unigenes 82个。另外,我们还预测到了9 287个微卫星(SSRs)。本研究为疣蝗基因功能分析、免疫通路相关基因和分子标记的进一步研究提供了分子理论基础。
Warts are widely distributed, is a common pest, the main hazard to crops, but the lack of molecular information. We obtained the unigenes from the wilt locust transcriptome using high-throughput Illumina sequencing and de novo assembly and identified innate immune-related signaling pathway genes. In this study, 86 756 unigenes were obtained from the wart locust transcriptome, with an average length of 670 bp and an N50 of 1 033 bp. There are 29 627, 13 866, 23 398, 13 474, 11 968, and 21 027 unigenes annotated for the unigenes of the warthog locus using the six databases Nr, Nt, Swiss-Prot, GO, COG and KEGG, respectively. In this study, we identified 319 unigenes related to innate immune signaling pathways, including 49 unigenes participating in the Toll pathway, 13 unigenes participating in the immunodeficiency (IMD) pathway, 117 unigenes participating in the JAK-STAT signaling pathway, There were 58 unigenes related to the fly pathway and 82 unigenes involved in the melanization pathway. In addition, we also predicted 9,287 microsatellites (SSRs). This study provided the molecular theoretical basis for the further study on gene function analysis of warts, loci related to immune pathways and molecular markers.