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探讨增强化疗药物抑制恶性肿瘤转移的新的有效方法。采用Ｃ５７ＢＬ／６小鼠右后肢腓肠肌内接种ｌｅｗｉｓ肺癌细胞制成实验动物模型，２１ｄ杀鼠取肺，计数肺转移结节数，观察丝裂霉素Ｃ（ＭＭＣ）化疗前预先７５ｍＧｙＸ射线全身照射对ＭＭＣ抑制肿瘤转移作用的影响。结果显示，单纯的３．０ｍｇ／ｋｇＭＭＣ腹腔注射不能明显减少ｌｅｗｉｓ肺癌的肺转移结节数（对照组：４１．７±７．４／肺，单纯化疗组：３３．８±１１．５／肺，Ｐ＞０．０５）；而化疗前６ｈ给予７５ｍＧｙＸ射线全身照射组肺转移结节数较单纯化疗组明显减少（２０．３±４．７／肺，Ｐ＜０．０５）。免疫学参数测定结果表明，化疗使荷瘤小鼠腹腔巨噬细胞（ＰＭΦ），脾脏自然杀伤细胞（ＮＫ）功能明显下降（Ｐ＜０．０５）；而７５ｍＧｙ＋ＭＭＣ组的免疫学参数较单纯化疗组显著提高（Ｐ＜０．０１）。提示化疗前预先７５ｍＧｙＸ射线全身照射对ＭＭＣ免疫抑制的减轻作用可能是其增强化疗抑制ｌｅｗｉｓ肺癌转移的重要基础。
To explore new and effective ways to enhance chemotherapeutic drugs in inhibiting the metastasis of malignant tumors. C57BL / 6 mice were inoculated with Lewis lung cancer cells in the right hindlimb gastrocnemius to establish experimental animal models. Rats were sacrificed on day 21 and the number of metastatic lung nodules was counted. Whole body irradiation of 75 mGy X-ray before mitomycin C (MMC) chemotherapy MMC inhibits tumor metastasis. The results showed that intraperitoneal injection of 3.0 mg / kg MMC alone did not significantly reduce the number of pulmonary metastases in lewis lung cancer (41.7 ± 7.4 / lung in the control group, 33.8 ± 11.5 / lung in the chemotherapy alone group , P> 0.05). Compared with the chemotherapy alone group, the number of lung metastases in the whole body irradiated with 75mGy X-rays 6h before chemotherapy was significantly decreased (20.3 ± 4.7 / lung, P <0.05). The results of immunological parameters showed that the function of peritoneal macrophages (PMΦ) and natural killer cells (NK) of tumor-bearing mice were significantly decreased by chemotherapy (P <0.05), while the immunological parameters of 75mGy + MMC group were significantly lower than those of chemotherapy alone group Significantly increased (P <0.01). It is suggested that the reduction of MMC immunosuppression by 75mGy X-ray whole body irradiation prior to chemotherapy may be an important basis for its enhancement of chemotherapeutic inhibition of lewis lung cancer metastasis.