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目的明确聚集性食源性腹泻暴发的病原。方法根据现场流行病学调查,对患者肛拭子标本18份,粪便标本2份,厨师粪便标本1份,进行分离培养,对分离到的可疑病原菌进行生化鉴定,采用Real-time PCR技术检测可疑病原菌的ipaH毒力基因,同时进行脉冲场凝胶电泳(PFGE)分型分析。结果分离到11株血清型为O136∶K78的肠侵袭性大肠埃希菌,除1份肛拭子外,其余7份患者肛拭子、3份粪便标本Real-time PCR均阳性;除1株菌外,其余10株菌株的PFGE带型一致。结论具有侵袭性相关基因ipaH的O136∶K78肠侵袭大肠埃希菌是本次聚集性腹泻暴发的主要原因。
Purpose To clarify the etiology of an episode of foodborne diarrhea. Methods According to the epidemiological survey in the field, 18 samples of anal swab, 2 stool samples and 1 chef stool sample were isolated and cultured. The suspicious pathogenic bacteria isolated were identified biochemically, and real-time PCR was used to detect suspicious Pathogenic bacteria ipaH virulence genes, while pulsed-field gel electrophoresis (PFGE) typing. Results 11 strains of Escherichia coli isolated from serogroups with O136: K78 serotypes were isolated. In addition to 1 rectal swab, the other 7 rectal swabs and 3 stool specimens were positive for Real-time PCR. The other 10 isolates had the same PFGE pattern. Conclusion O136: K78 intestinal invasive Escherichia coli with invasion-related gene ipaH is the main cause of this cluster of outbreaks of diarrhea.