全长 c DNA的获得是基因克隆的重要内容 ,也是目前人类基因组研究中的一个重要方面。 c DNA末端快速扩增 (RACE)技术是以 PCR为基础 ,从部分已知的 c DNA序列扩增出其他未知部分的 5′端和 3′端的 c DNA序列的一种方法。本文从 RACE技术的基本原理方法出发 ,详细阐述了其存在的缺陷 ,并对 RACE最新研究进展 ,如 Marathon- PCR、Smart- RACE以及“电子”c DNA克隆等作一综述 The acquisition of full-length c DNA is an important part of gene cloning and is also an important aspect in the present research of human genome. c DNA Rapid Amplification (RACE) is a PCR-based method to amplify 5 ’and 3’ c DNA sequences of other unknown parts from a partially known c DNA sequence. Based on the basic principles and methods of RACE technology, this paper elaborates its shortcomings and reviews the latest RACE research such as Marathon-PCR, Smart-RACE and “electronic” c DNA cloning