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对９个爪×籼Ｆ１杂种进行大规模花药培养，形成愈伤组织的花药的频率平均为１８．７％。小孢子衍生的愈伤组织产生绿苗的平均频率为８．７％。白化株占成苗愈伤组织的６１％。愈伤组织的绿株和白化株再生能力出现于花药培养的第４～８周之间；在第６周以后，白化株与绿株的比率没有增加．转移的愈伤组织成亩能力缓慢下降。自发的双单倍体占４个杂种再生绿苗的４６％。但在一杂种产生的１３２株双单倍体中发现了高频率的部分不育再生植株。
Large-scale anther culture of nine claw × indica F1 hybrids resulted in an average of 18.7% of anther callus formation. The average frequency of microspore-derived callus producing green shoots was 8.7%. Albino strains account for 61% of callus. The regeneration ability of calluses from green plants and albino plants appeared between the 4th and 8th week of anther culture. After 6 weeks, the ratio of albino and green plants did not increase. The transferred callus slowly decreased in capacity. Spontaneous double haploid accounted for 46% of four hybrid regeneration green seedlings. However, a high frequency of partially sterile regenerated plants was found in 132 doubled haploid hybrids.