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目的了解沈阳市2008—2009年流行性感冒(流感)流行季节H1N1亚型的HA1基因变异特征。方法利用沈阳市流感病毒H1N1亚型分离株,采用一步RT-PCR(反转录PCR)方法扩增HA1基因,测序,推到氨基酸序列,构建种系进化树。结果 2008—2009年共采集流感样病例咽拭子样本177份,共分离到流感病毒29株,分离率为16.4%。其中H1N1亚型21株。测定了10株H1N1亚型流感病毒分离株的HA1序列,与WHO推荐疫苗株A/B risbane/59/2007比对,同源性为94.2%~97.0%,氨基酸位点99、202、208、211、230发生了突变。结论沈阳市2008—2009年流感病毒发生了一定的变异,造成了该流行季节的H1N1亚型流感病毒流行。
Objective To understand the variation of HA1 gene in H1N1 subtype of epidemic season influenza (influenza) in Shenyang during 2008-2009. Methods H1N1 influenza virus H1N1 isolates were used to amplify HA1 gene by one-step reverse transcription-polymerase chain reaction (RT-PCR). The HA1 gene was sequenced and deduced to the amino acid sequence to construct phylogenetic tree. Results A total of 177 throat swab samples were collected during 2008-2009. A total of 29 strains of influenza virus were isolated, with a separation rate of 16.4%. Of which H1N1 subtype 21 strains. The HA1 sequences of 10 strains of H1N1 influenza viruses were determined and compared with the WHO recommended vaccine strain A / B risbane / 59/2007 with homology of 94.2% -97.0% and amino acid positions of 99,202,208, 211,230 mutation occurred. Conclusion The variation of influenza virus in Shenyang during 2008-2009 caused some epidemics of H1N1 subtype influenza virus in this epidemic season.