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目的 研究新型有机硒化合物Eb的组织分布特点、体外抗肿瘤及免疫活性 ,同时对其体内的抗肿瘤前景进行探讨。方法 昆明种小鼠连续灌胃给药Eb后 ,用荧光法测定心、肝、脾、肾、肺、胃、脑、肌、骨组织的硒含量 ,来考察Eb组织分布特异性。用MTT法检测其体外抗肿瘤活性。用淋巴细胞转化 ,溶血素抗体生成实验及吞噬细胞吞噬实验检测其免疫活性。结果 连续灌胃Eb治疗剂量 (0 1g·kg- 1 ·d- 1 ) 7天后 ,肝、脾、肺、肾、骨组织的硒含量增高 ,其中肝、脾组织的硒含量增高显著。心、胃、脑、肌肉组织的硒含量基本没变化 ;Eb对体外培养的人肝癌Bel 74 0 2细胞、胃癌BGC 82 3细胞、肺癌Calu 3细胞具有生长抑制作用 ;Eb具有促进淋巴细胞转化、溶血素抗体生成及增强吞噬细胞吞噬功能的作用。结论 Eb具有体外抗肿瘤 ,免疫增强活性及在肝、脾、肺、肾、骨组织的靶向性蓄积作用 ,表明Eb有作为抗肝、肺、肾、骨等组织肿瘤及增强免疫功能药物进一步研究的意义
Objective To study the tissue distribution characteristics of novel organic selenium compound Eb, anti-tumor and immunocompetence in vitro, and to explore the anti-tumor prospect in vivo. Methods Kunming mice were intragastric administration of Eb, and the distribution of selenium in heart, liver, spleen, kidney, lung, stomach, brain, muscle and bone was measured by fluorescence method. In vitro anti-tumor activity was detected by MTT assay. Lymphocyte transformation, hemolysin antibody production experiments and phagocytic phagocytosis test to test its immunocompetence. Results Selenium levels in liver, spleen, lung, kidney, and bone increased with continuous intragastric administration of Eb at a dose of 0 1 g · kg-1 · d-1 for 7 days. Selenium content in liver and spleen increased significantly. The selenium content of heart, stomach, brain and muscle tissue did not change basically. Eb had inhibitory effect on the growth of human hepatocellular Bel 7402 cells, gastric cancer BGC 82 3 cells and lung cancer Calu 3 cells in vitro. Eb could promote lymphocyte transformation, Hemolysin antibody production and enhance phagocytic phagocytosis. Conclusion Eb has anti-tumor and immunopotentiating activity in vitro and targeted accumulation in liver, spleen, lung, kidney and bone tissues, indicating that Eb has the potential as an anti-tumor, anti-tumor, The significance of research