环胞霉素A(CsA)是一种高效免疫抑制剂,它主要是在基因水平抑制T 细胞合成淋巴因子。本文发现CsA 是通过制淋巴细胞特异性因子与IL-2增强子(enhancer)结合的机制来抑制IL-2基因表达。作者将含有IL-2增强子—胸苷激酶(tk)启动子—CAT 结构基因的质粒转染入E14小鼠淋巴瘤细胞。TPA 和ConA 可诱导E14淋巴瘤表达CAT 基因,CsA 则抑制TPA和ConA 这种作用,而CsA 并不影响5× Cyclosporine A (CsA) is a potent immunosuppressive agent that inhibits T-cell lymphogenesis at the genetic level. Here we show that CsA inhibits IL-2 gene expression by binding to IL-2 enhancer by lymphocyte-specific factors. The authors transfected a plasmid containing the IL-2 enhancer-thymidine kinase (tk) promoter-CAT structural gene into E14 mouse lymphoma cells. TPA and ConA induced E14 lymphoma expression of CAT gene, CsA inhibited the role of TPA and ConA, while CsA did not affect 5 ×