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本文在建立稳定的离体灌注肾(IPK)装置基础上,利用一次性给予较大剂量环孢霉素A(CsA50μg/ml)引起IPK明显肾脏血流动力学障碍的模型,研究了内皮由来性舒张因子(EDRF)在CsA肾毒性发生中的意义。结果表明:正常IPK功能在本实验条件下能够稳定在2 h以上,CsA可以引起IPK RVR明显增高(P<0.05),RPF,GFR,UV,FF等明显下降(P分别小于0.01、0.05),SOD能通过加强EDRF的作用而改善由CsA引起的IPK血流动力学障碍,而EDRF抑制剂Hb可加强CsA的肾脏血流动力学效应。结果提示EDRF释放减少或灭活增多在CsA引起的IPK急性血流动力学障碍中可能具有重要意义。
Based on the establishment of a stable isolated perfused kidney (IPK) device, a model of obvious renal hemodynamic disorder caused by IPK 50 μg / ml in a single dose was given. Significance of Relaxation Factor (EDRF) in the Development of CsA Nephrotoxicity. The results showed that the normal IPK function could be stable for more than 2 h under the experimental conditions. CsA could significantly increase the RVR of IPK (P <0.05), and decrease the RPF, GFR, UV, FF and so on (P <0.01, SOD potentiates IPK hemodynamic disturbances caused by CsA by potentiating EDRF, whereas EDRF inhibitor Hb enhances renal hemodynamic effects of CsA. The results suggest that decreased EDRF release or increased inactivation may be of importance in CsA-induced IPK acute hemodynamic disorders.