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目的:探索法尼酯衍生物受体(farnesoid X receptor,FXR)在链脲佐菌素(Streptozotocin,STZ)诱导的1型糖尿病(diabetes mellitus,DM)大鼠模型回肠组织中的表达水平及意义。方法:将大鼠随机分为正常对照组(NC组)和DM模型组(DM组)。腹腔注射STZ建立DM大鼠模型,NC组予无水乙醇生理盐水溶液腹腔注射。通过实时荧光定量PCR检测FXR在回肠组织m RNA表达水平。结果:(1)DM组FXR m RNA表达量减少、糖化血红蛋白升高、平均血糖升高、体重下降与NC组之间的差异有统计意义(P<0.05);(2)FXR m RNA表达量pearson相关分析:DM组平均血糖、糖化血红蛋白、最终体重与最高体重相比下降的程度、最终体重与造模时体重相比下降的程度与FXR m RNA表达量呈负相关;(3)FXR m RNA表达量多元线性回归分析:DM组平均血糖、最终体重与最高体重相比下降的程度、糖化血红蛋白对FXR m RNA表达量的影响大(P<0.05),而NC组各个指标对FXR m RNA表达量无影响(P>0.05)。结论:FXR表达异常与糖尿病的发病和糖尿病患者的体重下降相关。
Objective: To investigate the expression of farnesoid X receptor (FXR) in ileum of diabetic mellitus (DM) rats induced by streptozotocin (STZ) and its significance . Methods: The rats were randomly divided into normal control group (NC group) and DM model group (DM group). The model of DM rats was established by intraperitoneal injection of STZ, and the rats in NC group were given intraperitoneal injection of saline solution of normal saline. Real-time fluorescence quantitative PCR was used to detect the mRNA expression of FXR in ileum. Results: (1) The expression of FXR m RNA in DM group was decreased, the HbA1c was increased, the average blood glucose was increased, the body weight was decreased and the difference was statistically significant (P <0.05); (2) pearson correlation analysis: The average level of blood glucose, glycosylated hemoglobin, the final body weight and the highest body weight in the DM group decreased, the final body weight and body weight decreased compared with the level of FXR m RNA expression was negatively correlated; (3) FXR m Multivariate linear regression analysis showed that the average blood glucose and the final body weight of DM group decreased compared with the highest body weight, and the effect of HbA1c on the expression of FXR m RNA was significant (P <0.05) No effect on expression (P> 0.05). Conclusion: The abnormal expression of FXR is associated with the onset of diabetes and weight loss in diabetic patients.