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目的观察丙型肝炎病毒(HCV)感染者血清中自身抗体情况,分析自身免疫反应在丙型肝炎中的作用。方法 100例丙型肝炎患者作为研究组,选取同时期体检中心进行体检的50例健康者作为对照组。以间接免疫荧光法(IIF)检测两组受检者血清中抗核抗体(ANA)、抗可提取性核抗原抗体(ENA)、免疫印迹法检测抗线粒体抗体(AMA)、抗肝抗原抗体(LMA);以实时荧光定量聚合酶链反应(PCR)法检测两组受检者血清丙型肝炎病毒核酸(HCV-RNA)含量。结果研究组中检测出1项或以上自身抗体阳性者48例,自身抗体阳性率为48.0%(48/100);对照组中检测出1项或以上自身抗体阳性者2例,自身抗体阳性率为4.0%(2/50)。研究组自身抗体阳性率显著高于对照组,差异有统计学意义(P<0.05)。研究组HCV-RNA阳性38例,自身抗体阳性23例(60.53%),研究组HCV-RNA阴性62例,自身抗体阳性25例(40.32%),对比差异有统计学意义(P<0.05)。结论 HCV感染后会出现自身免疫反应,且HCV-RNA阳性患者反应更明显,HCV感染者须进行血清自身抗体检测,以便更好接受治疗。
Objective To observe the autoantibodies in the serum of patients with hepatitis C virus (HCV) infection and to analyze the role of autoimmunity in hepatitis C virus. Methods One hundred patients with hepatitis C were selected as the research group. Fifty healthy subjects who took physical examinations during the same period were selected as the control group. Anti-nuclear antibody (ANA), anti-extractable anti-nuclear antigen antibody (ENA) were detected by indirect immunofluorescence assay (IIF) in serum, anti-mitochondrial antibody (AMA) LMA). The contents of HCV-RNA in serum of two groups were detected by real-time fluorescence quantitative polymerase chain reaction (PCR). Results 48 cases (48.0%) of the autoantibodies were detected in the study group (48/100). One or more autoantibodies were positive in the control group (2 cases), the autoantibody positive rate 4.0% (2/50). The positive rate of autoantibodies in the study group was significantly higher than that in the control group, the difference was statistically significant (P <0.05). HCV-RNA was found to be positive in 38 cases in the study group, with 23 cases of autoantibodies positive (60.53%). There were 62 cases with negative HCV-RNA in the study group and 25 cases (40.32%) with autoantibodies. The difference was statistically significant (P <0.05). Conclusion There is autoimmune reaction after HCV infection, and the reaction of HCV-RNA positive patients is more obvious. Serum autoantibodies should be tested in HCV infected patients in order to receive better treatment.