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目的 分析正常肺组织和肺癌组织的激光诱发自体荧光光谱 ,特征 ,探讨激光诱发自体荧光光谱区分正常肺组织和肺癌组织的可行性。方法 收集肺癌手术标本 39例 ,使用三倍频YAG激光(波长 35 5nm)和光学多道分析仪 (OMA)测定肺癌组织和正常肺组织的激光诱发荧光光谱 ,寻找其自体荧光光谱特征并与病理结果比较。结果 (1)正常肺组织和肺癌组织的主峰位置不同 [(471 6± 5 2 )nm对 (46 1 9± 4 3)nm],向右偏移 (P <0 0 0 1) ;(2 )肺癌组织的相对荧光强度大于正常肺组织 [(2 42 2 7± 434 9)对 (1485 4± 46 2 1) ](P <0 0 0 1) ;(3)正常肺组织在 5 6 0nm及 6 0 0nm处有 2个次峰 ,而肺癌组织平滑下降 ,正常肺组织在 5 80nm与 6 0 0nm的荧光强度比为 0 831± 0 178;肺癌为 1 2 6 9± 0 147(P <0 0 0 1)。结论 正常肺组织和肺癌组织的激光诱发自体荧光光谱有明显区别 ,可用于区分二者。
Objective To analyze the laser-induced autofluorescence spectra and characteristics of normal lung and lung cancer tissues and to explore the feasibility of laser-induced autofluorescence spectroscopy to distinguish normal lung tissues from lung cancer tissues. Methods Thirty-nine surgical specimens of lung cancer were collected. The laser-induced fluorescence spectra of lung cancer tissues and normal lung tissues were detected by triple frequency YAG laser (wavelength 35 5nm) and optical multiplex analyzer (OMA), and their autofluorescence spectrum characteristics were searched for. Results comparison. Results (1) The peak locations of normal lung tissue and lung cancer were different [(471 6 ± 5 2) nm vs. (46 1 9 ± 4 3) nm], shifted to the right (P 0 01) ) Lung cancer tissues were higher than those in normal lung tissues [(4242 ± 434 9) vs. (1485 4 ± 46 2 1)] (P <0.01); (3) And two sub-peaks at 600 nm, while the lung cancer tissues decreased smoothly. The fluorescence intensity ratio of the normal lung tissues at 5880nm and 60000 was 0 831 ± 0 178 and that of lung cancer was 1 269 ± 0 147 (P < 0 0 0 1). Conclusion Laser-induced autofluorescence spectra of normal lung and lung cancer tissues are obviously different and can be used to distinguish the two.