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目的建立检测人血浆利奈唑胺的HPLC法。方法以Eclipse XDB-C_(18)为色谱柱;甲醇-水(45:55,V/V)为流动相,流速1.0mL·min~(-1);血浆样品经70%高氯酸沉淀后,在254nm波长下进行检测。结果利奈唑胺在质量浓度0.25～50mg·L~(-1)内线性关系良好(r=0.999 8),定量下限为0.25mg·L~(-1);低、中、高浓度的日间、日内精密度(RSD)均<10%,相对回收率分别为96.40%、103.24%、100.33%,提取回收率>75%。结论本方法操作简便,结果准确,适用于血浆中利奈唑胺浓度的检测。
Objective To establish an HPLC method for the determination of linezolid in human plasma. Methods Eclipse XDB-C 18 column and methanol-water (45:55, V / V) as mobile phase at a flow rate of 1.0 mL · min -1. The plasma samples were precipitated with 70% perchloric acid , At 254nm wavelength detection. Results Linezolid had a good linearity (r = 0.999 8) with a lower limit of quantitation of 0.25 mg · L -1 in the concentration range of 0.25-50 mg · L -1. Low, medium and high concentrations of daytime , The intra-day precision (RSD) were less than 10%, the relative recoveries were 96.40%, 103.24% and 100.33%, respectively. The recovery rate was> 75%. Conclusion The method is simple, accurate and suitable for the determination of linezolid in plasma.