论文部分内容阅读
目的探讨P21ras、TBK1及bcl-xl在胰腺癌中的作用和其相互关系。方法应用免疫组化的方法检测P21ras、TBK1和bcl-xl在胰腺癌中的表达。结果 P21ras、TBK1和bcl-xl在胰腺癌中的表达率分别是76%、66%、60%;TBK1与P21ras、TBK1与bcl-xl呈正相关(P<0.05);P21ras与bcl-xl表达呈正相关(P<0.05),TBK1在p21ras阳性组织表达高于在p21ras阴性组织中的表达。TBK1和bcl-xl的过表达与肿瘤分化程度正相关,p21ras在不同分化程度的胰腺癌组织中有不同程度的表达,p21ras、TBK1和bcl-xl蛋白表达与胰腺癌淋巴结转移有明显相关。结论胰腺癌发生机制涉及ras信号通路中p21ras、TBK1与bcl-xl多个基因的异常,且具有协同作用,p21ras、TBK1与bcl-xl过表达在胰腺癌组织中均呈高表达,p21ras的表达与TBK1与bcl-xl的过表达正相关,提示K-ras基因突变可上调TBK1与bcl-xl的过表达,TBK1可能在K-ras基因突变诱导肿瘤发生发展中起了关键作用。
Objective To investigate the role and relationship of P21ras, TBK1 and bcl-xl in pancreatic cancer. Methods Immunohistochemistry was used to detect the expression of P21ras, TBK1 and bcl-xl in pancreatic cancer. Results The expression rates of P21ras, TBK1 and bcl-xl in pancreatic cancer were 76%, 66% and 60% respectively. The expressions of TBK1, P21ras and TBK1 were positively correlated with bcl-xl (P <0.05) (P <0.05). The expression of TBK1 in p21ras positive tissue was higher than that in p21ras negative tissue. The overexpression of TBK1 and bcl-xl was positively correlated with the degree of tumor differentiation. The expression of p21ras, TBK1 and bcl-xl in pancreatic cancer tissues with different degrees of differentiation was significantly correlated with lymph node metastasis of pancreatic cancer. Conclusions Pancreatic carcinogenesis involves in the abnormality of p21ras, TBK1 and bcl-xl genes in ras signaling pathway and has a synergistic effect. The overexpression of p21ras, TBK1 and bcl-xl are highly expressed in pancreatic carcinoma and the expression of p21ras The positive correlation between TBK1 and bcl-xl overexpression suggested that K-ras gene mutation could up-regulate the expression of TBK1 and bcl-xl. TBK1 may play a key role in the tumorigenesis induced by K-ras gene mutation.