系统性念珠菌感染是发病和死亡的重要原因,尤其是对免疫受损患者。而微生物学、组织学和血清学方法结合诊断该病的敏感性和特异性仍有待提高。聚合酶链反应(PCR)诊断真菌已广泛应用于多种临床标本如全血、血清、呼吸道分泌物、尿、腹腔积液和脑积液等。多数认为其敏感性高于血培养。除协助侵袭性念珠菌病的诊断外,一种理想的检测方法也应能帮助监测治疗反应以确定治疗时间。由于杀真菌剂的毒副作用较大,因此准确地评估停药时间对临 Systematic candidiasis is an important cause of morbidity and mortality, especially in immunocompromised patients. The sensitivity and specificity of microbiology, histology and serological methods to diagnose the disease remain to be improved. Polymerase chain reaction (PCR) diagnostic fungi have been widely used in a variety of clinical specimens such as whole blood, serum, respiratory secretions, urine, ascites and hydrocephalus. Most think its sensitivity is higher than blood culture. In addition to assisting in the diagnosis of invasive candidiasis, an ideal test should also help monitor the response to treatment to determine treatment time. Due to the greater toxicity and side effects of fungicides, it is important to accurately assess the stopping time