[Objective] To establish an optimal extraction process for simultaneous determination of seven effective metabolites of Magnolia officinalis Rendet Wils.By HPLC method.[Method] The chromatographic conditions were as follows:Samples were performed on the chromatographic column model Shim-pack CLC-ODS(4.6 mm×250 mm,5 m).The mobile phase consisted of acetonitrile(A) and 0.1% formic acid(B).[Result] The regression equation,with peak area(y) as ordinate and concentration(x) as abscissas,of chlorogenic acid,rutin,hyperin,quercitrin,quercetin,magnolol and honokiol,were y =1E+05x-306 360(r=0.999 8),y=5E+04x-79 920(r=0.999 4),y=5E+04x-55 280(r=0.999 9),y=5E+04x-60 530(r=0.999 9),y=2.5E+04x-57 010(r=0.999 8),y=2E+04x+7 102(r=0.999 9) and y=2E+04x+7 102(r=0.999 9),respectively.The concentrations of reference substances showed good linear relationship with peak area within 3.125-100.00 μg/ml.The average recovery rates of chlorogenic acid,rutin,hyperin,quercitrin,quercetin,magnolol and honokiol were 103.98%,101.65%,99.17%,103.59%,103.59%,102.44% and 104.62%,with RSD of 3.37%,2.86%,3.02%,2.89%,2.86%,1.18% and 3.67%,respectively.The content of chlorogenic acid,rutin,hyperin,quercitrin,quercetin,magnolol and honokiol from different parts of Magnolia offiinalis were different from each other.All the 7 effective compounds could be detected in the leaf of Magnolia offiinalis,thereinto,the content of quercitrin,magnolol and honokiol were high,while the content of hyperin and quercetin were low.[Conclusion] The established HPLC method was simple,convenient,stable and with good repeatability,which would provide reference for the further development and utilization of Magnolia officinalis Rendet Wils. [Objective] To establish an optimal extraction process for simultaneous determination of seven effective metabolites of Magnolia officinalis Rendet Wils.By HPLC method. [Method] The chromatographic conditions were as follows: Samples were performed on the chromatographic column model Shim-pack CLC-ODS (4.6 mm × 250 mm, 5 m). The mobile phase consisted of acetonitrile (A) and 0.1% formic acid (B). [Result] The regression equation, with peak area (y) as ordinate and concentration (x) as abscissas, of chlorogenic acid, rutin, hyperin, quercitrin, quercetin, magnolol and honokiol, were y = 1E + 05x- 306 360 (r = 0.999 8), y = 5E + 04x- 79 920 = 5E + 04x-55 280 (r = 0.999 9), y = 5E + 04x-60 530 (r = 0.999 9), y = 2.5E + 04x- 57 010 (r = 0.999 8), y = 2E + 04x +7 102 (r = 0.999 9) and y = 2E + 04x + 7 102 (r = 0.999 9), respectively. These concentrations of reference substances showed good linear relationship with peak area within 3.125-100.00 μg / ml. The average recovery rates of chlorogenic acid, rutin, hyperin, quercitrin, quercetin, magnolol and honokiol w ere 103.98%, 101.65%, 99.17%, 103.59%, 103.59%, 102.44% and 104.62% with RSD of 3.37%, 2.86%, 3.02%, 2.89%, 2.86%, 1.18% and 3.67% of chlorogenic acid, rutin, hyperin, quercitrin, quercetin, magnolol and honokiol from different parts of Magnolia offiinalis were different from each other. All the 7 effective compounds could be detected in the leaf of Magnolia offiinalis, thereinto, the content of quercitrin, magnolol and honokiol were high, while the content of hyperin and quercetin were low. [Conclusion] The established HPLC method was simple, convenient, stable and with good repeatability, which would provide reference for the further development and utilization of Magnolia officinalis Rendet Wils.