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目的 探讨用流式细胞术检测大肠癌淋巴结微转移的方法学及意义。方法 首先将大肠癌Lovo细胞以不同浓度加入到非大肠癌患者的淋巴结组织制备的细胞悬液中 ,经异硫氰酸荧光素(FITC)标记的细胞角蛋白 (Cytokeratin ,AE1/AE3)和碘化丙锭 (PI)双重染色后 ,用盲法进行流式细胞术检测 ,建立参照模型。然后以同样方法测定 2 5例大肠癌患者经常规病理检查转移阴性的 16 2个淋巴结。结果 参照模型对Lovo细胞的检出值与理论值呈较理想的一致性 (r=0 976 ) ,可以从 10 5个淋巴细胞中检出 10个癌细胞。在 7例 (2 8% )大肠癌患者的 38个 (2 3 45 % )常规切片诊断阴性的淋巴结中检到了癌细胞 ,并与癌大小和浸润深度有相关性。结论 流式细胞术检测大肠癌淋巴结微转移是一种可靠、快速及定量的理想方法。
Objective To investigate the methodology and significance of detecting lymph node micrometastasis in colorectal cancer by flow cytometry. Methods Colorectal cancer Lovo cells were first added to different concentrations of cell suspension prepared from lymph node tissue of patients with non-colorectal cancer. FITC-labeled cytokeratin (AE1 / AE3) and iodine After double staining of propidium iodide (PI), the flow cytometry was performed by blinded method to establish a reference model. Then, the same method was used to determine the 16 2 lymph nodes in 25 patients with colorectal cancer who were negative for routine pathological examination. Results The Lovo cells showed good agreement with the theoretical value (r = 0 976). Ten cancer cells could be detected from 105 lymphocytes. Cancer cells were detected in 38 (2345%) routinely diagnosed negative lymph nodes in seven (28%) patients with colorectal cancer and correlated with the size and depth of invasion. Conclusion Flow cytometry detection of lymph node micrometastasis in colorectal cancer is a reliable, rapid and quantitative ideal method.