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基于愈创木酚(GA)/葡萄糖(GS)/辣根过氧化物酶(HRP)/葡萄糖氧化酶(GOD)毛细荧光反应体系,以1,4-对苯醌为新型交联剂,将GOD固定在毛细管内表面上,开发了一种测定血糖的方法。经优选得到传感器连续测定反应液21次后,响应值仍有初始值的80%。保存在不同p H的PBS中(4℃),56 d后响应值仍有初始值的87%。此传感器测定GS的线性范围为1~12 mmol/L,检出限为0.82 mmol/L,RSD为4.2%(n=11),血清除蛋白后直接测定血糖,回收率为93.3%~108.4%。
Based on the capillary fluorescence reaction system of guaiacol (GA) / glucose (GS) / horseradish peroxidase (HRP) / glucose oxidase (GOD) GOD immobilized on the inner surface of the capillary developed a method of measuring blood glucose. After the sensor was continuously measured the reaction solution 21 times, the response value is still 80% of the initial value. After storage in PBS at different pH (4 ° C), the response was still 87% of the initial value after 56 days. The linear range of this sensor was 1 ~ 12 mmol / L, the limit of detection was 0.82 mmol / L and the RSD was 4.2% (n = 11). The blood glucose was measured directly after removing protein and the recovery was 93.3% -108.4% .