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目的通过观察慢性应激大鼠外周血促炎细胞因子的表达和肠道微生态的变化,探讨慢性应激对于其体内炎症和肠道微生物的影响。方法对大鼠序贯采用束缚、强迫游泳和噪声三种刺激48 d,分别将进行应激18、33、48 d时存活的大鼠作为慢性应激1组(n=20)、慢性应激2组(n=20)和慢性应激3组(n=20);将未受实验应激原刺激的20只大鼠作为正常对照组。检测并比较4组大鼠一般状况、外周血的促炎细胞因子和肠道微生态状况。结果对照组大鼠的饮食、精神与活动状态良好。3个慢性应激组大鼠均呈现食欲不好、活动减少、毛发无光泽、精神萎靡和对外界刺激反应迟钝,大鼠上述症状、体征随应激时间的延长而明显,即按正常组、慢性应激1、2、3组之序,症状、体征依次加重。随应激程度的递增(正常组→慢性应激1组→2组→3组),大鼠血清白细胞介素(IL)-1β、IL-6、肿瘤坏死因子(TNF)-α和干扰素(INF)-γmRNA相对表达量递升(P均<0.01);大鼠盲肠的肠杆菌和肠球菌定量[集落形成单位(CFU/g)]递升(P均<0.01),乳杆菌和双歧杆菌定量则递降(P均<0.01)。结论慢性应激可显著增加大鼠外周血促炎细胞因子水平和改变肠道微生态,变化程度与应激程度相关。降低促炎细胞因子水平和/或改善肠道微生态状态可能有利于改善其慢性应激状态。
Objective To observe the changes of proinflammatory cytokines and gut microflora in peripheral blood of chronic stress rats and to explore the effects of chronic stress on their inflammation and gut microbes. Methods The rats were subjected to three kinds of stimuli: swimming, swimming, and noise for 48 days. The rats that survived stress for 18, 33 and 48 days were divided into two groups: chronic stress group 1 (n = 20), chronic stress Twenty rats were randomly divided into two groups (n = 20) and chronic stress group (n = 20). Twenty rats which were not stimulated by experimental stress were used as normal control group. The general condition, peripheral blood proinflammatory cytokines and gut microflora were detected and compared. Results The rats in the control group had good diet, mental and activity status. The three chronic stress rats showed poor appetite, decreased activity, dull hair, apathetic and unresponsive to external stimuli, the above symptoms and signs of rats with stress prolonging and obvious, that is, according to the normal group, Chronic stress groups 1, 2, 3, symptoms, signs in turn increase. Serum interleukin (IL) -1β, IL-6, tumor necrosis factor (TNF) -α and interferon (IL-1β) were detected with increasing degree of stress (normal group → chronic stress group 1 → group 2 → group 3) (P <0.01). The amount of enterococci and enterococci in the cecum (CFU / g) increased gradually (P <0.01) Quantitative decline (P <0.01). Conclusion Chronic stress can significantly increase the levels of proinflammatory cytokines in peripheral blood and change the intestinal microflora. The degree of change correlates with the degree of stress. Reducing the level of proinflammatory cytokines and / or improving the intestinal microflora may be beneficial in improving their chronic stress state.