胆小管是两个相邻肝细胞膜细胞膜凹陷成槽并相互对接而成的微细管道 ,由于肝细胞胆小管面含有丰富的碱性磷酸酶 ,应用组化方法如钙—钴法等经典方法均能显示胆小管 [1 ,2 ,3 ] 。但由于这些方法步骤多 ,染色时间长 ,所需试剂较多 ,且容易褪色 ,因而有一定的局限性 ,我们利用原 Cholesteric tube is two adjacent cell membranes of the liver cell recessed into the groove and docking made of tiny tubes, the hepatobiliary surface is rich in alkaline phosphatase, the application of histochemical methods such as calcium - cobalt method Can show the bile duct [1, 2, 3]. However, due to the many steps of these methods, the dyeing time is long, the required reagent is more, and the color is easy to fade, so there are some limitations. We use the original