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目的探讨不同产地、不同等级掌叶大黄簇晶数与化学成分的相关性。方法采用定量分析法测定不同产地、不同等级掌叶大黄簇晶数,采用高效液相色谱(HPLC)法同时测定其中5种指标性成分(芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚)的含量,采用统计软件对得到的大黄簇晶数与5种指标性成分含量进行相关性分析。结果不同产地掌叶大黄簇晶数与5种指标性成分总含量及大黄素甲醚含量均呈显著正相关,同一产地、不同等级掌叶大黄簇晶数与5种指标性成分含量均呈显著正相关。结论测定大黄中簇晶数可作为其药材质量控制的手段之一,进而为大黄的质量控制提供新的技术手段,为其质量标准的制定提供一定的参考。
Objective To investigate the correlation between the chemical composition and the number of rhubarb clusters in different areas. Methods Quantitative analysis method was used to determine the number of rhubarb clusters in different origins and gradients of Rheum palmatum L. The five index components (aloe-emodin, rhein, emodin, chrysophanol and rhubarb The content of rhubarb cluster and the contents of five kinds of index components were analyzed by statistical software. Results The number of rhubarb clusters in different areas was positively correlated with the total content of five index components and the content of emodin. The number of crystal and the index of five index components of rhubarb were significantly different in the same place of production Positive correlation. Conclusion The determination of the number of clusters in rhubarb can be used as one of the means to control the quality of medicinal materials, and then provide a new technical means for the quality control of rhubarb, providing reference for the formulation of its quality standard.