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通过单因素试验研究了苯酚-硫酸法测定玫瑰枝叶可溶性多糖含量的最佳实验条件,即6%的苯酚溶液用量为1.0mL、浓硫酸用量为5.0mL,在室温下放置显色15min后于分光光度计488nm波长处测定吸光度。用此方法对生长季不同时期、不同生理状态(开花与未开花)下玫瑰枝、叶多糖含量进行了测定。试验结果显示:玫瑰叶片可溶性多糖含量高于枝条;开花植株的多糖含量高于未开花植株;在生长季不同时期玫瑰枝叶多糖含量呈现V形动态变化趋势。
The optimum experimental conditions for the determination of soluble polysaccharide content in rose leaves and leaves by phenol-sulfuric acid method were studied by single factor experiment, that is, the amount of 6% phenol solution was 1.0mL, the concentration of sulfuric acid was 5.0mL, Photometric 488nm wavelength measured absorbance. Using this method, the contents of polysaccharides in the branches and leaves of roses were determined at different growth seasons and in different physiological states (flowering and not flowering). The results showed that the content of soluble polysaccharides in rose leaves was higher than that of shoots. The content of polysaccharides in flowering plants was higher than that in non-flowering plants. The content of polysaccharides in rose leaves showed V-shape dynamic trend in different growth periods.