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测定人心肌肌凝蛋白（Ｍｙｏｓｉｎ）、肌凝蛋白轻链（Ｍ－ＬＣ）、重链（Ｍ－ＨＣ）的ＡＴＰ酶活性，结果Ｍ－ＬＣ的Ｍ－ＬＣＡＴＰ酶活性略高于肌凝蛋白ＡＴＰ酶活性，Ｍ－ＨＣ无ＡＴＰ酶活性。同时采用ＥＬＩＳＡ方法测定９６例正常人及４７例心肌梗塞病人，结果正常人血清Ｍ－ＬＣ１平均值为５．９６ｎｇ／ｍｌ，心肌梗塞病人为７３．５ｎｇ／ｍｌ（Ｐ＜０．００１）。心肌酶谱与Ｍ－ＬＣ１对心肌梗塞诊断符合率比较，Ｍ－ＬＣ１诊断符合率为１００％，ＣＰＫ同功酶ＭＢ为９０．６％。本组制备的单抗与兔心肌肌凝蛋白无交叉反应，抗鸡心肌Ｍ－ＬＣ的单抗与本组提纯的Ｍ－ＬＣ１亦无亲和反应，说明人心肌Ｍ－ＬＣ１单抗具有种属特异性。
The activities of ATPases in Myosin, M-LC and M-HC were determined. The M-LCATP activity of M-LC was slightly higher than that of Myosin ATP Enzyme activity, M-HC No ATPase activity. Meanwhile, 96 normal subjects and 47 myocardial infarction patients were detected by ELISA. The average serum M-LC1 was 5.96ng / ml in normal subjects and 73.5ng / ml in patients with myocardial infarction (P <0.001). The coincidence rate between myocardial enzymes and M-LC1 in diagnosing myocardial infarction was 100% for M-LC1 and 90.6% for CPK isoenzyme MB. This group prepared monoclonal antibody and rabbit cardiac myosin no cross-reaction, anti-chicken M-LC monoclonal antibody and the group of purified M-LC1 also no affinity reaction, indicating that human myocardial M-LC1 monoclonal antibody species Specificity.