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本研究旨在观察不同强度运动时 ,与能量代谢有关的骨骼肌腺嘌呤核苷酸 (ATP)、磷酸肌酸 (CP)、乳酸 (LA)和呼吸链氧化还原的变化特点 ,以进一步阐明训练中运动强度这一重要因素的生理意义。雄性SD大鼠先经 5周耐力训练筛选 ,再进行 3周递增速度训练。正式实验时 ,随机分成 6个强度组 (0m/min、18m/min、30m/min、4 2m/min、5 4m/min、6 6m/min) ,6只 /组 ,每组以 6m/min的增幅进行 3分钟准备活动 (0m/min组为安静时处死 ) ,然后以预定强度跑 3分钟后立即处死 ,取右腿用液氮固定 ,凿取腓肠肌 ,经冷冻干燥后制备肌肉提取液。用高效液相法测定肌肉ATP、ADP、AMP、CP、NADH和NAD+ ,用紫外分光法检测肌乳酸。结果表明 ,随着运动强度的增加 ,运动后即刻大鼠腓肠肌能量代谢产物的变化如下 :(1)ATP浓度在 18～ 4 2m/min范围内呈下降趋势 ,超过 4 2m/min出现上升趋势 ,其中 ,4 2m/min组比安静组降低 (P <0 0 1) ,5 4m/min组较 4 2m/min组升高 (P <0 0 5 ) ;ADP和AMP的变化表现为波动性上升的趋势 ;CP浓度、能荷 (EC)以及ATP/ADP的比值呈波动性下降趋势 ;同时 ,随着ATP/ADP比值的下降 ,AMP的生成增加 ,CP逐渐降解。(2 )肌乳酸、NADH和NADH/NAD+ 比值呈波动性上升趋势 ,当强度大于 4 2m/min时NADH/NAD+明显上升 ,
This study aimed to observe the changes of skeletal muscle adenine nucleotide (ATP), creatine phosphate (CP), lactic acid (LA) and respiratory chain redox associated with energy metabolism at different intensities of exercise to further clarify the training In the exercise intensity of this important factor of physiological significance. Male Sprague-Dawley rats were screened for endurance training for 5 weeks and then for 3 weeks of increasing speed training. Formal experiments were randomly divided into 6 intensity groups (0m / min, 18m / min, 30m / min, 42m / min, 54m / min, 66m / min) (0 m / min group was executed at rest), and then sacrificed for 3 minutes at a predetermined intensity. The right leg was fixed with liquid nitrogen, the gastrocnemius muscle was chiselled, and the muscle extract was prepared by lyophilization. Muscle ATP, ADP, AMP, CP, NADH and NAD + were measured by high performance liquid chromatography, and myoglobin was detected by UV spectrophotometry. The results showed that with the increase of exercise intensity, the changes of energy metabolites in the gastrocnemius muscle of rats immediately after exercise were as follows: (1) The ATP concentration showed a downward trend in the range of 18-4 2m / min, Among them, 42m / min group was lower than that of quiet group (P <0.01), 54m / min group was higher than 42m / min group (P <0 05), ADP and AMP showed a fluctuating increase (P <0.05). The concentrations of CP, EC and ATP / ADP showed a decreasing trend. At the same time, with the decrease of ATP / ADP ratio, the production of AMP increased and CP gradually degraded. (2) The ratios of myo-lactic acid, NADH and NADH / NAD + showed a fluctuating upward trend. NADH / NAD + increased significantly when the intensity was greater than 42 m / min,