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目的探讨磷脂酰肌醇-3激酶(PI3K)抑制剂对高糖环境下肾小球足细胞上皮间质转分化(EMT)的影响。方法体外培养原代大鼠肾小球足细胞,分为NG组(葡萄糖5mmol/L)、MG组(甘露醇30 mmol/L)、HG组(葡萄糖30 mmol/L)、HG+LY294002组(葡萄糖30 mmol/L+LY294002 2mg/L),培养48h后检测α-平滑肌肌动蛋白(α-SMA)和E-cadherin蛋白的表达情况。结果 HG组足细胞足突消失及失去细胞间连接。与NG组比较,HG组α-SMA蛋白表达增多,E-cadherin蛋白表达减少(P<0.05);与HG组相比,HG+LY294002组α-SMA蛋白表达减少,E-cadherin蛋白表达增加(P<0.05)。结论高糖导致足细胞损伤发生EMT,PI3K抑制剂可部分阻断EMT的发生。
Objective To investigate the effects of phosphatidylinositol 3-kinase (PI3K) inhibitors on epithelial-mesenchymal transition (EMT) of glomerular podocytes in high glucose environment. Methods The primary rat glomerular podocytes were cultured in vitro and divided into 3 groups: NG group (glucose 5mmol / L), MG group (mannitol 30mmol / L), HG group (glucose 30mmol / L), HG + LY294002 group Glucose 30 mmol / L + LY294002 2 mg / L) for 48 hours. The expression of α-smooth muscle actin (α-SMA) and E-cadherin protein were detected. Results HG group foot cell foot process disappeared and lost the connection between cells. Compared with NG group, α-SMA protein expression and E-cadherin protein expression were decreased in HG group (P <0.05); Compared with HG group, α-SMA protein expression and E-cadherin protein expression were increased in HG + LY294002 group P <0.05). Conclusion High glucose led to EMT in podocyte injury, PI3K inhibitor may partially block the occurrence of EMT.