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为了筛选抗原封闭液中聚乙二醇(PEG4000)的适宜浓度,首先用狂犬病病毒(Rabies virus,RV)细胞培养物包被抗原板,以含1%BSA和5%蔗糖的PBST溶液中加入不同浓度的PEG作为封闭液,分别于4℃、37℃保存7,14,30,90,180 d,从中筛选含适宜浓度PEG的封闭液,然后分别用乙肝表面抗原(HBsAg)、猪瘟病毒E2抗原(CSFV E2)和猪繁殖与呼吸综合征nsp2基因(PRRSV nsp2)原核表达产物作为包被抗原,对所筛选出的封闭液进行稳定性评价。结果表明:当封闭液为含12%PEG+1%BSA+5%蔗糖的PBST时,上述4种抗原包被板均可在37℃稳定保存180 d。由于该封闭液对不同来源抗原所具有的良好稳定性,初步推断该封闭液可能具有较广泛的应用前景。
To screen suitable concentrations of PEG4000 in the antigen blocking solution, antigen plates were first coated with Rabies virus (RV) cell culture and PBST solutions containing 1% BSA and 5% sucrose Concentration of PEG as a blocking solution, respectively, at 4 ℃, 37 ℃ for 7,14,30,90,180 d, screening containing the appropriate concentration of blocking solution of PEG, and then respectively with hepatitis B surface antigen (HBsAg), swine fever virus E2 antigen CSFV E2) and porcine reproductive and respiratory syndrome nsp2 gene (PRRSV nsp2) as coating antigen, and the stability of the selected blocking solution was evaluated. The results showed that when the blocking solution was PBST containing 12% PEG + 1% BSA + 5% sucrose, the above four antigens were stably stored at 37 ° C for 180 days. Due to the good stability of the blocking solution to antigens from different sources, it is preliminarily concluded that the blocking solution may have a broader application prospect.