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本文用苏木精染色和双苯并咪唑(Hoechst 33258)染色法,从草菇子实体“纽期”菌褶分化完开始,每3小时对同一个子实体连续切取菌褶进行染色观察。结果表明草菇子实体“纽期”菌褶形成时,约10％的担子发生了核配;在子实体发育过程中,尤其是子实体成熟期后,不断有少量新的双核担子产生,并发生核配,使草菇减数分裂的同步性不高;草菇从菌褶分化完成(此时已有10％担子发生核配)到子实体完全成熟,菌褶变成深粉红至褐色(此时约70％担子完成减数分裂)需要28—30小时;担子减数分裂的持续时间为18小时,其中细线期和偶线期5.9小时、粗线期6.2小时、双线期和终变期3.4小时、中期10.5小时、后期Ⅰ到四分体2小时;经过对粗线期、双线和终变期以及中期Ⅰ染色体条数的多次反复观察,认为草菇的染色体条数为11(n=11);减数分裂后,4个子核分别进入4个担孢子中,留下无核的担子;绝大部分担孢子是单核的,有约5％的担孢子是双核的。
In this study, hematoxylin staining and bisbenzimidazole (Hoechst 33258) staining method was used to observe the follicles of the same fruiting bodies continuously cut off every 3 hours from the beginning of the follicular differentiation of “New Zealand” of Agrobacterium fruiting bodies. The results showed that about 10% of the basidiomycetes occurred during the formation of the Pleurotus ostreatus, and a small amount of new dual-core burden occurred continuously during the development of the fruiting body, especially after the fruiting body matured The occurrence of nuclear distribution, so that the synoptis meiosis synchronization is not high; straw mushroom differentiation from the fold to complete (at this time there is 10% of the burden of nuclear distribution) to fruiting bodies fully mature, gill folds into a deep pink to brown About 70% of the burden is now meiosis) takes 28-30 hours; the duration of meiosis of the burden is 18 hours, of which 5.9 days for fine line and even line, 6.2 hours for thick line, Variable period of 3.4 hours, 10.5 hours of medium, late I to four hours of 2 hours; after the pachytene, double and the end of the metaphase Ⅰ and chromosome number repeatedly observed that the number of straw mushroom chromosome 11 (n = 11). After meiosis, the four sub-nuclei entered into four basidiospores respectively, leaving a non-nuclear burden. Most of the basidiospores were mononuclear and about 5% of the basidiospores were dinuclear .