目的　采用逆转录 聚合酶链反应 (RT PCR)的方法从少量外周血中扩增抗HBsAg抗体重链Fd和κ轻链基因。方法　用RNAex试剂提取细胞总RNA ,以Olig (dT)引导合成cDNA第一链 ,再经PCR扩增的方法 ,以重链Fd和κ轻链基因简并引物 ,直接从人外周血混合淋巴细胞中扩增出抗HBsAg抗体重链Fd和κ轻链基因。 结果　扩增出分子量为 70 0bp的抗HBsAg抗体重链Fd和κ轻链基因。 结论　得到的抗体基因为下一步构建含人抗HBsAg抗体Fab段基因的腺相关病毒载体奠定了基础 Objective To amplify the anti-HBsAg heavy chain Fd and κ light chain genes from a small amount of peripheral blood by reverse transcription-polymerase chain reaction (RT PCR). Methods RNAex reagent was used to extract the total RNA of cells. Olig (dT) was used to guide the synthesis of the first strand of cDNA. Then the PCR products were amplified by PCR. The dendritic cells were transfected directly with human peripheral blood mixed lymphocytes In the amplification of anti-HBsAg antibody heavy chain Fd and kappa light chain genes. As a result, the anti-HBsAg antibody heavy chain Fd and kappa light chain genes with a molecular weight of 70 0 bp were amplified. Conclusions The obtained antibody gene lays a foundation for further construction of adeno-associated virus vector containing human anti-HBsAg Fab fragment gene