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目的研究明日叶查尔酮对2型糖尿病大鼠肝脏葡萄糖转运体2(Glut2)和骨骼肌葡萄糖转运体4(Glut4)蛋白表达的影响。方法制造2型糖尿病大鼠模型,随机分成高、中、低剂量组和糖尿病对照组,分别每日灌胃给予30、10、5和0mg/kg BW明日叶查尔酮。各组以高脂饲料喂养4周后,用葡萄糖氧化酶法检测空腹血糖;放射免疫法检测血清胰岛素含量;免疫组化法检测葡萄糖转运体蛋白表达水平。结果经图像分析,高剂量组肝脏和骨骼肌葡萄糖转运体蛋白表达平均光密度值分别为(0.036±0.0030)和(0.063±0.0139),均较糖尿病对照组显著升高(P<0.05)。高剂量组空腹血糖和胰岛素水平分别为(12.3±1.64)mmol/L和(25.65±3.34)μIU/ml,均较糖尿病对照组显著性降低(P<0.05)。结论明日叶查尔酮可上调2型糖尿病大鼠肝脏葡萄糖转运体2和骨骼肌葡萄糖转运体4蛋白表达水平,降低空腹血糖和胰岛素水平,改善糖尿病胰岛素抵抗状况。
Objective To investigate the effect of echinactrone on the expression of hepatic glucose transporter 2 (Glut2) and skeletal muscle glucose transporter 4 (Glut4) in type 2 diabetic rats. Methods A rat model of type 2 diabetes mellitus was established. The rats were randomly divided into high, medium and low dose groups and diabetic control group. The mice were administered 30, 10, 5, and 0 mg / kg BW of metronidazole chelate daily. The rats in each group were fed with high-fat diet for 4 weeks, then the fasting blood glucose was detected by glucose oxidase method; the content of serum insulin was detected by radioimmunoassay; the protein expression of glucose transporter was detected by immunohistochemistry. Results The average optical density of hepatic and skeletal muscle glucose transporter protein in the high-dose group was (0.036 ± 0.0030) and (0.063 ± 0.0139) respectively, which were significantly higher than those in the diabetic control group (P <0.05). The fasting blood glucose and insulin levels were (12.3 ± 1.64) mmol / L and (25.65 ± 3.34) μIU / ml in the high dose group, respectively, which were significantly lower than those in the diabetic control group (P <0.05). Conclusion Yechakone could up-regulate the hepatic glucose transporter 2 and skeletal muscle glucose transporter 4 protein expression in type 2 diabetic rats, reduce fasting blood glucose and insulin levels, and improve insulin resistance in diabetic rats.