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目的:探究RAS相关的C3肉毒素底物1(RAC1)在胃癌中的表达及其与临床病理学特征的相关性,并观察RAC1对胃癌细胞的增殖、迁移等生物学特性的影响。方法:使用免疫组织化学染色方法研究RAC1在胃癌中的表达情况。分别使用χ2检验及Kaplan-Meier生存分析的统计学方法分析RAC1的表达与胃癌临床病理学的特征及预后的相关性。并探究高表达RAC1在体外实验中对胃癌细胞生长、迁移、侵袭的影响。结果:RAC1在胃癌组织中高表达,且不同分化程度、有无局部侵袭、淋巴结转移和不同Lauren’s分型间均有差异(均P<0.05)。RAC1高表达预示了胃癌患者的不良预后。在胃癌细胞中诱导RAC1高表达后发现其在体外促进胃癌细胞的生长、迁移及侵袭(均P<0.05)。使用特异性抑制剂抑制RAC1表达后发现,胃癌细胞生长、迁移及侵袭的生物学功能也受到相应的抑制(均P<0.05)。结论 :RAC1与胃癌的侵袭性相关且是预后不良的指标。体外实验结果也进一步证实了RAC1高表达与胃癌细胞的侵袭性相关,鉴于其在胃癌生长及进展中所起的重要作用,RAC1可作为胃癌治疗的潜在靶点。
Objective: To investigate the expression of RAS-associated C3 Botulinum toxin 1 (RAC1) in gastric cancer and its relationship with clinicopathological features and to observe the effects of RAC1 on the biological characteristics of gastric cancer cells such as proliferation and migration. Methods: The expression of RAC1 in gastric cancer was studied by immunohistochemical staining. The relationship between the expression of RAC1 and clinicopathological features and prognosis of gastric cancer was analyzed by χ2 test and Kaplan-Meier survival analysis respectively. And to explore the effect of RAC1 highly expressed on the growth, migration and invasion of gastric cancer cells in vitro. Results: RAC1 was highly expressed in gastric cancer tissues, with different degrees of differentiation, with or without local invasion, lymph node metastasis and different Lauren’s classification (all P <0.05). High RAC1 expression predicts poor prognosis in patients with gastric cancer. RAC1 overexpression in gastric cancer cells was found to promote gastric cancer cell growth, migration and invasion in vitro (all P <0.05). Inhibition of RAC1 expression using specific inhibitor found that the biological function of gastric cancer cells growth, migration and invasion were also inhibited (all P <0.05). Conclusion: RAC1 is associated with gastric cancer aggressiveness and is an indicator of poor prognosis. The results of in vitro experiments further confirmed that the high expression of RAC1 is associated with the invasiveness of gastric cancer cells. Given its important role in the growth and progression of gastric cancer, RAC1 may serve as a potential target for the treatment of gastric cancer.