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目的:研究转化生长因子β1(TGF-β1)对人成熟树突状细胞(m DCs)生物物理特性与迁移能力的影响。方法:用不同浓度TGF-β1(1μg/L、3μg/L、5μg/L、7μg/L)处理m DCs 24 h,按TGF-β1浓度将m DCs分为4组和对照组(未加入细胞因子);采用细胞渗透脆性实验检测m DCs的渗透脆性(未破碎细胞百分率),通过细胞电泳实验测定m DCs电泳率,荧光偏振实验和微吸管系统分别测定m DCs荧光片振度和被吸入管内的长度,以反映m DCs细胞膜的流动性和变形性,利用Transwell系统检测m DCs跨内皮细胞迁移百分率。结果:与对照组相比,(1)细胞渗透脆性,在1μg/L组m DCs破碎百分率增加,5μg/L组m DCs破碎百分率降低,差异有统计学意义(P<0.05);(2)细胞电泳率,1μg/L和3μg/L组m DCs电泳率下降,差异有统计学意义(P<0.05或P<0.01);(3)细胞膜流动性和细胞变形性,细胞膜流动性在5μg/L组增加;变形能力在5μg/L和7μg/L组增加,差异有统计学意义(P<0.01或P<0.05);(4)细胞迁移能力,1μg/L和3μg/L组m DCs跨内皮细胞迁移率下降,差异有统计学意义(P<0.05或P<0.01)。结论:TGF-β1通过影响m DCs的生物物理特性从而抑制其跨内皮细胞迁移的能力。
Objective: To investigate the effect of transforming growth factor-β1 (TGF-β1) on the biophysical properties and migration ability of human mature dendritic cells (m DCs). Methods: m DCs were treated with different concentrations of TGF-β1 (1μg / L, 3μg / L, 5μg / L, 7μg / L) for 24 h. The m DCs were divided into 4 groups according to the concentration of TGF- Factor). The osmotic fragility (unbroken cell percentage) of m DCs was measured by cell infiltration fragility test. The electrophoretic activity of m DCs was measured by cell electrophoresis experiment. Fluorescence polarization experiment and micro-pipette system were used to measure the vibrational degree of m DCs fluorescent plate, To reflect the fluidity and deformability of the membrane of m DCs. Transwell system was used to detect the percentage migration of m DCs across endothelial cells. Results: Compared with the control group, (1) the cell infiltration fragility increased with the increase of the number of m DCs in 1μg / L group and the percentage of m DCs in 5μg / L group, the difference was statistically significant (P <0.05); (2) Cell electrophoresis rate, electrophoretic rate of m DCs in 1μg / L and 3μg / L groups decreased significantly (P <0.05 or P <0.01); (3) Cell membrane fluidity and cell deformability, cell membrane fluidity at 5μg / (P <0.01 or P <0.05). (4) The migration ability of cells, the number of m DCs in 1μg / L and 3μg / L group were significantly higher than those in L group The endothelial cell migration rate decreased, the difference was statistically significant (P <0.05 or P <0.01). CONCLUSION: TGF-β1 inhibits its ability to migrate across endothelial cells by affecting the biophysical properties of m DCs.