AIM:To investigate the inhibitory effect of dietary andmedicinal formula Ganfujian granule on diethylnitrosamine(DEN)-induced hepatocarcinoma in rats.METHODS:Male SD rats had free access to water containing0.1 g/L DEN for 16 weeks,during which the rats fed withstandard diet or administration of Ganfujian granule(30.4 g/Kgin diet).At weeks 4,8,12 and 16 of hepatocarcinogenesis5 rats of each group were sacrificed,and at week 20 another30 rats were sacrificed from each group.The end point forsurvival observation was at week 28.Immunochemistrymethods were used to examine the effect of Ganfujiangranule on the process of hepatocarcinogenesis includingproliferation of hepatocytes and cell cycle modulation.RESULTS:Ganfujian granule could reduce and delay theincidence of hepatocarcinoma in rats and prolong the survivalof animals.In addition,Ganfujian granule had a markedinhibitory effect on high expression of cyclin dependent kinase(CDK4)during the whole process of hepatocarcinogenesisand cyclin D1 at week 16 and the number of proliferatingcell nuclear antigen(PCNA)positive cells in different stagesof hepatocarcinogenesis.CONCLUSION:Ganfujian granule can reduce and delaythe incidence of hepatocarcinoma in rats by exerting director indirect effects on cell cycle and inhibiting uncontrolledproliferation of hepatocytes. AIM: To investigate the inhibitory effect of dietary and medicinal formula Ganfujian granule on diethylnitrosamine (DEN) -induced hepatocarcinoma in rats. METHODS: Male SD rats had free access to water containing 0.1 g / L DEN for 16 weeks, during which the rats fed Acceptable diet or administration of Ganfujian granule (30.4 g / Kgin diet) .At weeks 4, 8, 12 and 16 of hepatocarcinogenesis 5 rats were sacrificed, and at week 20 another 30 rats were sacrificed from each group. The end point forsurvival observations was at week 28. Immunochemistry methods were used to examine the effect of Ganfujiangranule on the process of hepatocarcinogenesis includingproliferation of hepatocytes and cell cycle modulation .RESULTS: Ganfujian granule could reduce and delay theincidence of hepatocarcinoma in rats and prolong the survivalof animals.In addition, Ganfujian granule had a marked effect on high expression of cyclin dependent kinase (CDK4) during the whole process of hepatocarcinogenesis and cycli n D1 at week 16 and the number of proliferating cell nuclear antigen (PCNA) positive cells in different stages of hepatocarcinogenesis. CONCLUSION: Ganfujian granule can reduce and delay the incidence of hepatocarcinoma in rats by exerting director indirect effects on cell cycle and inhibiting uncontrolled proliferation of hepatocytes.