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目的:观察人类巨细胞病毒(human cytomegalovirus,HCMV)作用于血管内皮细胞(vein endothelialcells,ECV)对E选择素时序性的表达,探讨HCMV感染致动脉粥样硬化(atherosclerosis,AS)发生和发展的可能机制。方法:用HCMV感染ECV,采用RT-PCR方法检测不同感染时段细胞E选择素mRNA及蛋白的表达。结果:HCMV感染ECV后,感染0 h时E选择素mRNA有基础水平的表达,4 h升高并达高峰,8 h时开始回落,12 h回落显著,24 h回落更明显,与0 h比较,差异均有统计学意义(P<0.01),48 h接近0 h表达水平(P>0.05)。结论:HCMV感染ECV后能够促进E选择素表达,并且呈时间依赖性。HCMV有可能通过上调E选择素的表达,介导ECV的炎症反应,促进AS的发生、发展。
OBJECTIVE: To observe the time-dependent expression of E-selectin by human cytomegalovirus (HCMV) on vascular endothelial cells (ECV) and to investigate the occurrence and development of atherosclerosis (AS) induced by HCMV infection Possible mechanism. Methods: ECV was infected with HCMV, and the expression of E-selectin mRNA and protein was detected by RT-PCR. Results: After ECV infection with HCMV, the expression of E-selectin mRNA at 0 h was up-regulated at 4 h, then peaked at 8 h, then retreated significantly at 12 h, and then declined more significantly at 24 h , The difference was statistically significant (P <0.01), 48 h close to 0 h expression (P> 0.05). Conclusion: HCMV can promote the expression of E-selectin after ECV infection in a time-dependent manner. HCMV may increase the expression of E-selectin, mediate the inflammatory response of ECV and promote the occurrence and development of AS.