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利用各种色谱技术从贵州天名精的丙酮提取部位分离得18个倍半萜内酯化合物。通过全面的光谱数据鉴定了它们的结构,涉及2个卡拉布烷型倍半萜天名精内酯酮(1),4R-天名精内酯醇(2);3个桉烷型倍半萜大叶土木香内酯(3),3-epi-isotelekin(4),1α-hydroxypinatifidin(5);8个愈创木烷型倍半萜[4β,10α-dihydroxy-5α(H)-1,11(13)-guaidien-8α,12-olide(6),8-epi-helenium lactone(7),4-epi-isoinuviscolide(8),9β,10β-epoxy-4α-hydroxy-1β-H,11α-H-guaian-8α,12-olide(9),4α,10α-dihydroxy-1β(H),5β(H)-guaian-11(13)-en-8α,12-olide(10),4α-hydroxy-9β,10β-epoxy-11β-H,5α-H-guaian-11(13)-en-8α,12-olide(11),4α-hydroxy-1β,5α,11α-H-guaian-9(10)-en-8α,12-olide(12),inuviscolide(13)],1个伪愈创木烷型倍半萜[(+)-confertin(14);3个吉马烷型倍半萜madolin B(15),carabrolactone A(16),11(13)-去氢腋生依瓦菊素(17);及1个苍耳烷型倍半萜tomentosin(18),其中,化合物1的绝对构型用X-射线单晶衍射法确认,而化合物2的C-4位手性中心的绝对构型用改良mosher法确定。化合物2~5,7,9~15均为首次从本种分离得到,而其中化合物4~5,7,12~15又为首次从本属植物分离得到。另外,采用大鼠嗜铬细胞瘤细胞(PC12细胞)为研究模型,在细胞形态观察和细胞分化率统计学分析的基础上评价了化合物1~3,6和17的神经营养活性,但研究结果显示它们均无活性。
A total of 18 sesquiterpene lactone compounds were isolated from the acetone extract of P. japonicus using various chromatographic techniques. Their structures were identified by comprehensive spectroscopic data, involving 2 carbitol sesquiterpenes (1), 4R-pendimethalinol (2), 3 eucalyptus sesqui (3), 3-epi-isotelekin (4), 1α-hydroxypinatifidin (5); 8 guaiacylpenoid sesquiterpenes [4β, 10α-dihydroxy-5α (13) -guaidien-8α, 12-olide (6), 8-epi-helenium lactone (7), 4-epi- isoinuviscolide (8), 9β, 11α-H-guaian-8α, 12-olide (9), 4α, 10α-dihydroxy-1β (H), 5β (H) -guaian-11 (13) -hydroxy-9β, 10β-epoxy-11β-H, 5α-H-guaian-11 (13) -en-8α, 12-olide (11) (10) -en-8α, 12-olide (12), inuviscolide (13)] and one pseudogeneticin-type sesquiterpene [ Terpenoids madolin B (15), carabrolactone A (16), 11 (13) -dehydrogenated axyridin (17); and one xanthane type sesquiterpenoid tomentosin (18) The configuration was confirmed by X-ray single crystal diffractometry, while the absolute configuration of the C-4 chiral center of Compound 2 was confirmed by a modified mosher method. Compounds 2 ~ 5, 7, 9 ~ 15 were isolated from this species for the first time, and compounds 4 ~ 5,7,12 ~ 15 were isolated from this genus for the first time. In addition, using rat pheochromocytoma cells (PC12 cells) as a model, the neurotrophic activities of compounds 1 to 3, 6 and 17 were evaluated on the basis of morphological observation and statistical analysis of cell differentiation rates. However, the results Show that they are inactive.