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为了研究同种异体成骨细胞与钙磷陶瓷复合的体内成骨性能，分别在５只新西兰白兔的臀部两侧皮下和肌内植入单纯羟基磷灰石、双相磷酸钙陶瓷材料和复合骨膜来源成骨细胞的羟基磷灰石、双相磷酸钙陶瓷材料。４周后取材作组织学、体视学及材料内成分分析（ＥＤＸＡ法）。结果显示：①材料内均有结缔组织长入，无明显炎性反应。②单纯材料内无成骨，复合材料内有成骨，并证实有Ｃａ、Ｐ沉着。③不同植入部位及不同复合材料内成骨量无显著差异，但肌内成骨呈板层状，而皮下成骨仅呈矿化组织。因而认为：①复合成骨细胞的钙磷陶瓷具有良好的生物相容性，并有明显优越的成骨性能。②同种异体成骨细胞经酶消化法提纯并传代培养后，其抗原性明显降低，并仍保持成骨能力。③丰富的血循环可能有利于新生骨更趋成熟。复合成骨细胞的钙磷陶瓷材料有可能是一类极具应用前景的骨缺损修复替代材料
In order to study the in vivo osteogenic properties of allogeneic osteoblasts and calcium-phosphorus ceramic composite, hydroxyapatite, biphasic calcium phosphate ceramic materials and composites were subcutaneously and intramuscularly implanted into the buttocks of five New Zealand white rabbits respectively Periosteum-derived osteoblasts hydroxyapatite, biphasic calcium phosphate ceramic material. Four weeks later, the specimens were taken for histology, stereology and material composition analysis (EDXA method). The results showed that: ① materials have connective tissue growth, no obvious inflammatory reaction. ② simple material without osteogenesis, composite material within the bone, and confirmed Ca, P calm. ③ There was no significant difference in the amount of osteogenesis between different implant sites and different composite materials, but the intramuscular osteoblasts were lamellar, while the subcutaneous osteogenesis was only mineralized. So that: ① composite osteoblast calcium phosphate ceramics have good biocompatibility, and has significantly superior osteogenic properties. ② After allogeneic osteoblasts were purified by enzyme digestion and subcultured, the antigenicity of the allogeneic osteoblasts was significantly reduced and still maintain the osteogenic capacity. ③ rich blood circulation may be conducive to newborn bone more mature. Calcium phosphate ceramic composite osteoblasts may be a very promising alternative bone defect repair materials