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目的 :探讨从血液中早期检出伤寒沙门氏菌病原体的方法。方法 :选择沙门氏菌属中的invAinvE ,采用PCR扩增 -反相杂交法及电泳法同时对血培养物中的伤寒沙门氏菌进行对比检测。结果 :电泳法 6h检出 8份 ,检出率 80 %。 8h、10h可将 10份全部检出 ,检出率为 10 0 %。反相杂交法 4h有 3份阳性 ,阳性率为 30 % ,6h有 9份出现阳性反应 ,阳性率为 90 % ,8h、10h可将 10份全部检出 ,阳性率为 10 0 %。临床标本中电泳法 12 / 16阳性 ,阳性率 75 % ,PCR -反相杂交法阳性 14/ 16 ,阳性率87.5 %。结论 :反相杂交法具有敏感性高、特异性好等优点 ,对伤寒的早期快速诊断及治疗有重要意义。
Objective: To explore the early detection of pathogen of Salmonella typhi in the blood. Methods: InvAinvE from Salmonella was selected and Salmonella typhi in blood culture was detected by PCR amplification-reverse hybridization and electrophoresis simultaneously. Results: Electrophoresis 6 h detected 8 copies, the detection rate of 80%. 8h, 10h 10 can be all detected, the detection rate of 10 0%. In the reverse hybridization method, 3 were positive at 4h, the positive rate was 30%, and the positive rate was 9 at 6h. The positive rate was 90%. 10h after 10h, the positive rate was 10%. The positive rate of clinical samples was 12/16, the positive rate was 75%, the positive rate of PCR - reverse hybridization was 14/16, the positive rate was 87.5%. CONCLUSION: Reversed-phase hybridization has the advantages of high sensitivity and good specificity, which is of great significance to the early diagnosis and treatment of typhoid fever.