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目的:建立双波长RP-HPLC法同时测定牛黄上清片中黄芩苷和栀子苷含量的方法。方法:色谱柱:Hibor C18柱(4.6mm×150 mm,5μm),流动相:乙腈-0.2%磷酸水溶液,采用梯度洗脱,检测波长:240 nm(栀子苷),278 nm(黄芩苷),柱温:30℃。结果:栀子苷和黄芩苷进样量分别在0.16~0.96μg(r=0.9999),0.18~1.08μg(r=0.9999)范围内线性关系良好,平均加样回收率分别为95.6%,96.0%,RSD分别为2.7%,2.5%。结论:该法简便、快速、灵敏,可用于牛黄上清片中栀子苷和黄芩苷的含量测定。
Objective: To establish a dual wavelength RP-HPLC method for the simultaneous determination of baicalin and geniposide in bezoar supernatant. METHODS: The chromatographic column was Hibor C18 column (4.6 mm × 150 mm, 5 μm). The mobile phase consisted of acetonitrile-0.2% phosphoric acid aqueous solution with gradient elution. The detection wavelength was 240 nm for geniposide and 278 nm for baicalin. Column temperature: 30 ° C. Results: The calibration curves of geniposide and baicalin were linear within the range of 0.16-0.96 μg (r = 0.9999) and 0.18-1.08 μg (r = 0.9999), respectively. The average recoveries of geniposide and baicalin were 95.6% and 96.0% , RSD respectively 2.7%, 2.5%. Conclusion: The method is simple, rapid and sensitive and can be applied to the determination of geniposide and baicalin in bezoar supernatant tablets.