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引言本文选择二个例子作为生物合成核苷酸的模式反应。第一个例子是Tochikura等人用啤酒酵母(Saccharomyces cerevisiae)细胞从一磷酸腺苷(AMP)生产三磷酸腺苷(ATP)。图1表明,为了从1克分子AMP产生1克分子ATP,必须从2克分子二磷酸腺苷(ADP)再生2克分子ATP,而酵母细胞的糖酵解过程起一个ATP再生器的作用。第二个例子是Nakayma和Hagino和Tochikura等人报道的用啤酒酵母从一磷酸胞苷(CMP)和氯化胆碱生产二磷酸胞苷-胆碱(CDPCholine)。
Introduction Two examples were chosen as the model reactions for biosynthetic nucleotides. The first example is the production of adenosine triphosphate (ATP) from adenosine monophosphate (AMP) by Tochikura et al. Using Saccharomyces cerevisiae cells. Figure 1 shows that in order to produce 1 mole of ATP from 1 mole of AMP, 2 moles of ATP must be regenerated from 2 moles of adenosine diphosphate (ADP), whereas the yeast cell’s glycolysis process acts as an ATP regenerator. The second example is the production of CDPCholine from S. monocytosine (CMP) and choline chloride using S. cerevisiae as reported by Nakayma and Hagino and Tochikura et al.